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cd96 apc  (Miltenyi Biotec)


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    Miltenyi Biotec cd96 apc
    Cd96 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd96+apc/us12345712-1605-33-34?v=Miltenyi+Biotec
    Average 93 stars, based on 12 article reviews
    cd96 apc - by Bioz Stars, 2026-08
    93/100 stars

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    CD3−CD45+CD56+ NK cells were evaluated side by side in PBMCs of healthy donors (HD) and melanoma patients (MP) and in metastatic melanoma (MM) using flow cytometry. (A) Pooled data showing the frequencies of NK cells within CD3−CD45+ cells (n=16 per group). (B) Representative dot plots showing the percentages of TIGIT and CD226 expression by NK cells. (C) Pooled data of TIGIT and CD226 expression (% and MFI) by NK cells in PBMCs (HD: n=30 and n=25, respectively; MP: n=39 and n=32, respectively) and MM (n=17 and n=14, respectively). (D) TIGIT/CD226 expression ratio (MFI) by NK cells (from panel C). (E) Percentages of CD96, PD-1, Tim-3, NKG2D, CD16, 2B4, NKp46, NK30 and CD57 expression by cNKs isolated from HDs (n=9–12) and MPs (n=7–20), and TiNKs (n=10–11). Horizontal bars depict means and P values were obtained by one-way ANOVA tests followed by Tukey’s multiple comparisons test with *, P<0.05; **, P<0.01; ***, P<0.001 and ****, P<0.0001. Data are representative of ten independent experiments.

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: IL-15 stimulation with TIGIT blockade reverses CD155-mediated NK cell dysfunction in melanoma

    doi: 10.1158/1078-0432.CCR-20-0575

    Figure Lengend Snippet: CD3−CD45+CD56+ NK cells were evaluated side by side in PBMCs of healthy donors (HD) and melanoma patients (MP) and in metastatic melanoma (MM) using flow cytometry. (A) Pooled data showing the frequencies of NK cells within CD3−CD45+ cells (n=16 per group). (B) Representative dot plots showing the percentages of TIGIT and CD226 expression by NK cells. (C) Pooled data of TIGIT and CD226 expression (% and MFI) by NK cells in PBMCs (HD: n=30 and n=25, respectively; MP: n=39 and n=32, respectively) and MM (n=17 and n=14, respectively). (D) TIGIT/CD226 expression ratio (MFI) by NK cells (from panel C). (E) Percentages of CD96, PD-1, Tim-3, NKG2D, CD16, 2B4, NKp46, NK30 and CD57 expression by cNKs isolated from HDs (n=9–12) and MPs (n=7–20), and TiNKs (n=10–11). Horizontal bars depict means and P values were obtained by one-way ANOVA tests followed by Tukey’s multiple comparisons test with *, P<0.05; **, P<0.01; ***, P<0.001 and ****, P<0.0001. Data are representative of ten independent experiments.

    Article Snippet: The following conjugated mAbs were used in flow cytometry experiments: TIGIT-PerCPeFluor710, TIGIT-PE, CD14-PacificBlue and CD19-PacificBlue (ThermoFisher Scientific), NKG2D-PE-Cy7, PD-1-PE-Cy7 and CD226-PE-Dazzle594 (Biolegend), CD96-APC and Tim-3-Alexa700 (R&D Systems), CD45-BV510, CD56-BUV395, CD8-BUV737, CD3-APC-Cy7, and CD3-FITC (BD), HLA-DR-ECD and CD16-PE-Cy7 (Beckman Coulter), CD226-PE or CD226-biotin (DX11, Abcam) coupled with streptavidin-PE-TexasRed (ThermoFisher Scientific) and/or IgG control mAbs.

    Techniques: Flow Cytometry, Expressing, Isolation